Antimicrobial activity of stable silver nanoparticles of a certain size
Identifieur interne : 002160 ( Main/Exploration ); précédent : 002159; suivant : 002161Antimicrobial activity of stable silver nanoparticles of a certain size
Auteurs : Iu. P. Mukha [Ukraine, Niger] ; A. M. Eremenko [Ukraine] ; N. P. Smirnova [Ukraine] ; A. I. Mikhienkova [Ukraine] ; G. I. Korchak [Ukraine] ; V. F. Gorchev [Ukraine] ; A. Yu. Chunikhin [Ukraine]Source :
- Applied Biochemistry and Microbiology [ 0003-6838 ] ; 2013-03-01.
Abstract
Abstract: Conditions for obtaining stable silver nanoparticles smaller than 10 nm were developed using a binary stabilizer polyvinylpyrrolidone/sodium dodecylsulphate in optimal ratio. Optical spectra, morphology and dependence of size of the nanoparticles on the amount of reducing agent were studied. Colloidal solutions of nanosilver showed a high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. The mechanism of action of nanosized silver on microbial cell was examined by laser scanning confocal microscope using fluorescent label. First step of antimicrobial effect on microorganisms was membrane damage and penetration of silver nanoparticles into the cell. Prolonged stability of nanoparticles and their antimicrobial activity over the past two years were showed.
Url:
DOI: 10.1134/S0003683813020117
Affiliations:
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Marzeev Institute for Hygiene and Medical Ecology NAMS Ukraine, 02660, Kyiv-94</wicri:regionArea><wicri:noRegion>Kyiv-94</wicri:noRegion></affiliation></author><author><name sortKey="Gorchev, V F" sort="Gorchev, V F" uniqKey="Gorchev V" first="V. F." last="Gorchev">V. F. Gorchev</name><affiliation wicri:level="1"><country xml:lang="fr">Ukraine</country><wicri:regionArea>A. V. Palladin Institute of Biochemistry NAS Ukraine, 01601, Kiev-601</wicri:regionArea><wicri:noRegion>Kiev-601</wicri:noRegion></affiliation></author><author><name sortKey="Chunikhin, A Yu" sort="Chunikhin, A Yu" uniqKey="Chunikhin A" first="A. Yu." last="Chunikhin">A. Yu. Chunikhin</name><affiliation wicri:level="1"><country xml:lang="fr">Ukraine</country><wicri:regionArea>A. V. 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Optical spectra, morphology and dependence of size of the nanoparticles on the amount of reducing agent were studied. Colloidal solutions of nanosilver showed a high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. The mechanism of action of nanosized silver on microbial cell was examined by laser scanning confocal microscope using fluorescent label. First step of antimicrobial effect on microorganisms was membrane damage and penetration of silver nanoparticles into the cell. Prolonged stability of nanoparticles and their antimicrobial activity over the past two years were showed.</div></front></TEI><affiliations><list><country><li>Niger</li><li>Ukraine</li></country></list><tree><country name="Ukraine"><noRegion><name sortKey="Mukha, Iu P" sort="Mukha, Iu P" uniqKey="Mukha I" first="Iu. P." last="Mukha">Iu. P. 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